BIOSYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING CLEOME VISCOSA LEAVES EXTRACT AND EVOLUTION OF ANTIBACTERIAL ACTIVITY

M. Indumathi, Dr. A. Subramaniyan

Abstract

Green synthesis of silver nanoparticles was successfully done using Cleome viscosa leaf extract by simple, rapid, eco-friendly and a cheaper method. In this study, silver nanoparticles were synthesized using leaf extract from C. viscosa, which facilitated the reduction of silver nitrate into silver ions. The obtained AgNPs were characterized by UV, FTIR, XRD and SEM-EDX analysis. The presence of biosynthesized AgNPs was confirmed by UV-visible spectroscopy and also crystal nature of AgNPs confirmed through XRD analysis; FT-IR spectrum was used to confirm the presence of different functional groups in the biomolecules which act as a reducing and capping agent for the nanoparticles. The morphology of the synthesized silver nanoparticles (AgNPs) was examined using SEM, and their silver content was verified through elemental analysis. The green-synthesized AgNPs displayed effective antibacterial activity against both Gram-negative and Gram-positive bacteria.